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View Full Version : giant microbes!!!!!


Tuburculosis
09-20-2006, 03:39 PM
the record for most members on line is 21.lets make it bigger!4.30 tommorow in easten tome(new york,eastern sea borrd)figure out your own time!instead of 21,we can make it over 100!

mazuac
09-20-2006, 04:30 PM
OH... okay???

Pollock II
09-20-2006, 04:34 PM
Too bad none of the users really show up anymore. I'll try to get on, if I don't have class or work or anything.

mazuac
09-20-2006, 04:36 PM
i will try to get on, i highly doubt i will be able to though

Tuburculosis
09-20-2006, 05:19 PM
yes.i hope we can.for the benifit ofeople at schoool,6.30.

kskerr
09-20-2006, 05:59 PM
I will likely be in seminar or something, maybe lab.

mazuac
09-20-2006, 06:29 PM
lol, how is everybody's microbes?

kskerr
09-20-2006, 06:33 PM
lol, how is everybody's microbes?

My normal flora seem to be doing fine, I have plates in the incubator and some sitting out so their color will develop (I use a reagent that causes my bacteria to turn blue while others remain white, it is $450 for 1 gram so I call it blue gold), as far as I know they are all doing fine, though I do not like how some of the plates on the bench came out smeary instead of clear, makes counting the number of bacteria on the plate difficult and inaccurate.

Tuburculosis
09-20-2006, 06:39 PM
lets stay on topic.remembr!

kskerr
09-20-2006, 08:24 PM
lets stay on topic.remembr!

If that is directed at me I did, I commented on my microbes. If you meant my plushes well they are not living and so are as they always are.

Pollock II
09-20-2006, 11:47 PM
My normal flora seem to be doing fine, I have plates in the incubator and some sitting out so their color will develop (I use a reagent that causes my bacteria to turn blue while others remain white, it is $450 for 1 gram so I call it blue gold), as far as I know they are all doing fine, though I do not like how some of the plates on the bench came out smeary instead of clear, makes counting the number of bacteria on the plate difficult and inaccurate.


Please tell me you watch Mythbusters and saw the episode on fecal particles on toothbrushes!

kskerr
09-21-2006, 02:26 AM
Please tell me you watch Mythbusters and saw the episode on fecal particles on toothbrushes!

Well not the way I would recommend to get your dose of Bifidobacteria but to each their own I guess...

I love that show but rarely get a chance to watch it, do not watch much tv in general. Could you recap it for those of us who sadly missed it...

Pollock II
09-21-2006, 10:11 AM
They put 100 toothbrushes in different parts of a bathroom to see if the myth "keeping your toothbrush next to the toilet will get poop on your toothbrush" was true.

They also kept another toothbrush in an entirely different area.

After the 2 weeks of this, they found that all of the toothbrushes, including the one NOT kept in the bathroom had fecal particles on it.

Pollock II
09-21-2006, 02:46 PM
Microbes = the topic of this whole forum. You're railroaded hundreds of threads with random stuff. And how can a thread that's one true purpose is telling people to show up on a certain day really be able to stay on the topc of "Let's all show up on this day". All the people who actually post on this board have already said that they're pretty sure they're unable to come due to previous engagements.

kskerr
09-21-2006, 03:03 PM
They put 100 toothbrushes in different parts of a bathroom to see if the myth "keeping your toothbrush next to the toilet will get poop on your toothbrush" was true.

They also kept another toothbrush in an entirely different area.

After the 2 weeks of this, they found that all of the toothbrushes, including the one NOT kept in the bathroom had fecal particles on it.

I question their detection method(s) then, feces are not really an aerosol and so I do not see how they could travel all over the bathroom and out of the bathroom. What was or were the detection method(s)?

mazuac
09-21-2006, 03:06 PM
i wish i could grow microbes...

Pollock II
09-21-2006, 03:12 PM
I question their detection method(s) then, feces are not really an aerosol and so I do not see how they could travel all over the bathroom and out of the bathroom. What was or were the detection method(s)?

They used the toothbrushes for 2 weeks, and then rubbed them on petree dishes to see if they had fecal stuff on them. They called in some science researcher who was well trained to do it.

Pollock II
09-21-2006, 03:15 PM
i wish i could grow microbes...


Go to your local science/ hobby store and ask them for some agar gel. Follow the directions to prepare it, the wander around your house with scotch tape!

Take the tape and stick it to something that you think has LOTS of germs on it (door knobs, toilet seats, kitchen and bathroom counter tops). Make sure you remember where each bit of tape was from in your house. Stick the tape on teh agar gel to transfer the assumed germs from the tape to the gel.

Store them in a dark place and wait. Soon you'll have things growing on your agar! It's really cool and really fun!

Tuburculosis
09-21-2006, 03:25 PM
its more people than i thought!wow!

kskerr
09-22-2006, 01:46 AM
They used the toothbrushes for 2 weeks, and then rubbed them on petree dishes to see if they had fecal stuff on them. They called in some science researcher who was well trained to do it.

That is hilariously flawed from the sounds of it. They were looking at bacteria on something that sat out exposed to air and was put in someone's mouth repeatedly for 2 weeks, hahahaha!!! Even assuming the used selective media to try for kinds that are normally associated with feces it is absurd. Most fecal associated bacteria can be found in the environment as well and not just in feces. Generally the mythbusters are great and design wonderful experiments but I think this one is not one of them. The fact that their control (the toothbrush(es) that were outside the bathroom) came out positive should have raised an eyebrow.

Personally I would look for bile or something that is only found coming out of the colon and not in the mouth, the chemical route most likley. Can't look for DNA or cells because those are found in the mouth, unless it were cell types from the colon (not sure how different they are though).

If you want to use microbial methods you would have to find a way of ensuring that the bacteria on the brush came from feces, most likely the feces of the person(s) using that bathroom, perhaps label those bacteria somehow, but most methods would also label the oral flora as well, plus a popular labeling method is using radiolabeling and feeding people radioactive bacteria is generally not considered a good idea... Hmm perhaps some genetically engineered E. coli that glows in the dark on special media, I made that once, though they would have to use a different kind than I did since mine was designed to not live outside of a very specific environment (to prevent it from colonising us students, not that it would have likley hurt us, but it did contain antibiotic resistance genes we added as well to help select for it, very common practice but those genes could possibly transfer to a pathogen in the gut and cause issues...).

kskerr
09-22-2006, 02:07 AM
i wish i could grow microbes...

You do all the time ;), there are microbes on your skin (staphylococcus is common), in your mouth (many kinds often including spirochetes, the spiral shaped kind), in your throat (streptococcus, mutant strains cause strep throat but most are not harmful), hopefully not in your stomach since the only one I know of is one that causes ulcers, and in your gut (much of the space is filled with them). You could also collect left over food and watch it rot, fungi and/or bacteria are the things that rot food, just don't let it stink too bad, I doubt your mother would like this idea too much...

Pollock's idea is actually very good and what I was going to suggest before she beat me to it, I have seen a kit at hobby lobby that had some sort of agar (there is agar which is the solidifying agent and it is normally added to some sort of broth that contains nutrients and such for them to eat), it also came with a petri dish. If you lived by me I could have given you some old plates but oh well. Generally plastic ones are considered disposable but as you would get a limited number you will likely want to clean and reuse them, I have boxes with I think 500 in them. If you do this I suggest taking a Q-tip and rubbing a tooth and then gently spreading it across the agar and seeing what grows, you will want to put it in a warm spot but not too hot or you will fry them, and yes they generally like it dark. You could also take a q-tip to raw meat, that should have some interesting bugs on it, behind your ear should have a nice collection of staphylococcus, they might grow for you. Oh dirt, yes, that is a great one, it has all kinds of wonderful things in it. If you have any salad greens around they would be fun, take and gently press a leaf to the surface, take it off and see if anything comes grows, should grow in the shape of the leaf if you are careful. Use your imagination!